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樣品均質破碎

Bertin Precellys Evolution多功能組織均質器

法國Bertin Precellys Evolution生物樣品均質器集樣品研磨、細菌胞體裂解、流體均質和組織勻漿功能為一體。工作頭8字型三維振動帶動樣品管內研磨珠密集撞擊和剪切,在約30秒時間完成對樣品高效處理。選配Cryolys Evolution冷卻系統,可確保樣品勻漿期間溫度維持在 0 - 10℃。

設備名稱:組織均質器 組織勻漿儀 珠式研磨儀Tissue Homogenizer

制 造 商:Bertin Technologies公司    產 地:法國

品      牌:Bertin

型      號:Precellys Evolution

技術細節:見[技術規格]    商品報價:見[訂貨指南]

主要功能用途
法國Bertin Precellys Evolution是一款集固體樣品研磨、細菌胞體裂解、流體均質和組織勻漿為一體的多功能生物樣品均質器。通過工作頭的8字型三維振動(可達10000rpm),帶動樣品管內研磨珠(玻璃珠、陶瓷珠、鋼珠等)高速振蕩,借助于密集撞擊和剪切力,能在大約30秒時間里實現對樣品研磨、裂解、均質處理??捎糜谒熊浗M織(細菌、肝臟、大腦、皮膚等)、堅硬/堅韌的組織(骨頭、玉米、毛發、種子、土壤等)、微生物(革蘭氏陽性菌、革蘭氏陰性菌、酵母)樣品、糞便、土壤等的可靠處理。


技術特點
Precellys Evolution均質器工作頭帶動樣品管垂直方向3維震動,比單一水平或垂直方向振蕩研磨方式對樣品破碎均質更高效,普通樣品10-40s即可完全均質。而樣品處理時間大幅縮短,可有效防止樣品溫度的大幅上升,減少溫度敏感型樣品的損傷??赏瑫r處理24個2ml/1.5ml/0.5ml或12個7ml或者6個15ml生物樣品,每個樣品單管封閉操作,避免交叉污染。而大型觸摸屏程序編程控制,直觀易用,進一步提高批間、批內樣品處理效果的均一性和穩定性。
1、人體工程學設計的樣品管上樣
采用專利真空壓蓋技術加樣品管固定鎖板,固定樣品管無需平衡,即插即用,上樣更方便、快捷。
密封O-型皮樣品室及
研磨艙及電機內部的連接部分用O-型橡膠環密封圈隔絕,確保樣品管發生破裂時液體及樣品控制在樣品艙內部,防止樣品污染的擴散到系統主機內部,方便清理,安全可靠。
真空壓蓋技術運用,降低了樣品管高速振蕩過程氣動產熱,減少樣品溫度上升程度。
真空壓蓋技術、壓力及過熱警報和強化保護罩, 適合在生物安全三級 (BSL3)實驗室操作使用,可用于朊病毒樣品(瘋牛癥) 檢測。

2、堅硬樣品處理經濟型解決方案
特制金屬7ml 管及夾板 (選配) 針對堅硬難磨樣本,樣品管可以重復使用,有效降低使用成本并確保研磨效果。

3、完善的操作安全保護機制
多重安全保障(壓力/溫度/速度/蓋子/電源報警)保障使用者和實驗室的安全。
3種安全防護:過溫報警、壓力報警、開蓋報警。

4、有效的溫度控制
研磨設定時間的最大設定值為90秒,避免客戶過度研磨,樣品不要過度升溫。
研磨工序內置暫停時間,確保樣品熱量減少,防止持續工作快速升溫對樣品損壞。
對樣品溫度要求極為苛刻的用戶,有Cryolys Evolution冷卻系統供選配,可維持工作室內的低溫狀態。
內置溫度報警功能。

5、Cryolys Evolution冷卻系統
Cryolys Evolution嵌入式冷卻模塊是Bertin公司專為Precellys Evolution均質器處理熱敏感生物樣本提供獨特控溫保護開發的新一代冷卻裝置。
Cryolys Evolution具有高效冷卻功能,可中和常規樣品勻漿過程中所產生熱量,確保在Precellys Evolution樣品勻漿期間樣品艙內溫度維持在 0 - 10℃,防止癌癥研究、藥理學實驗等樣品RNA、siRNA、代謝產物、酶或蛋白質等熱敏感分子(thermosensitive molecules)的受熱加速降解,確保獲得高產量提取。
Cryolys Evolution使用液氮揮發或干冰升華作為冷媒,-50℃的低溫冷氣自然沉降進入樣品工作室,包圍每個樣品管,確保均質化過程中的樣品內溫度保持在0-10℃之間。
Cryolys Evolution可從Precellys Evolution主機控制界面,通過系統集成控制軟件進行編程控制冷卻流量可調。借助于溫度探頭,可通過Precellys Evolution軟件監控精確的溫度。
Cryolys Evolution無需壓縮機制冷裝置,直接安裝于Precellys Evolution均質器頂部,通過Precellys直接供電。無需任何空氣管路或電源線,不占用額外工作臺面,空間局促或生物安全性實驗室也可以正常使用。

 

技術規格

Precellys   Evolution均質器

樣品工作容量

24×2 mL/0.5 mL

12×7mL

6×15mL

鎖定系統

自動管鎖系統 (automatic tubes locking system)

轉速范圍

4500~10000rpm(以100 rpm遞增設定)

可設定工作程序數

允許保存無限數量的協議

可編程循環次數(Number   of cycles)

每個程序最高可設10個循環

循環時間長(Cycles   time)

每個循環運行時間為1-90秒,1秒遞增可調

周期之間暫停(Pause   between cycles)

循環之間間隔1-120秒,1秒遞增可調

操作期間噪音

小于70dB

主機尺寸(W×D×H)

515×378×295mm(開蓋時515mm)

主機重量

27.2Kg

認證合格

CE, FCC part 15 & 18

Cryolys   Evolution冷卻系統

樣品艙控溫范圍

0   - 10 ℃

制冷方式

液氮或干冰提供冷源

干冰容積

1.5kg干冰

干冰消耗量

1.5kg干冰可工作30 min (run time)

尺寸(W×D×H)

335×335×100mm

重量

2.6kg

 

安裝運行條件

工作電源:90 - 250VAC/50 - 60 Hz,1 kW

允許環境溫度:5 – 40℃

允許相對濕度:≤80%

 

系統組成

Precellys Evolution組織均質器的基本工作部件包主機、樣品管工作套件、研磨管及研磨珠耗材。

對樣品處理過程有低溫要求的實驗,可選配Cryolys Evolution冷卻單元。

品名

品牌

貨號

規格

描述

報價

24×2mL樣品管工作套件

Bertin

S002274-PEVO0-A

each

2mL   holder pack for Precellys Evolution

Contains:

1x 2mL white blocking plate

1 x 2mL tube holder

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12×7mL樣品管工作套件

Bertin

S000911-PEVO0-A

each

7mL   holder pack for Precellys Evolution

Contains:

1 x 7mL grey blocking plate

1 x 7mL tubes holder

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12×7mL金屬管工作套件

Bertin

S002275-PEVO0-A

each

Metal tube holder pack for Precellys Evolution

Contains:

1 x Metal tube blocking plate for Precellys Evolution

1 x Metal tube holder for Precellys Evolution

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6×15mL樣品管工作套件

Bertin

S000810-PEVO0-A

each

15mL   holder pack for Precellys Evolution

Contains:

1x 15mL blue blocking plate

1x 15mL tubes holder

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96孔樣品工作套件

Bertin

S002276-PEVO0-A

each

96-Well Plate holder pack V2 for Precellys Evolution

Contains:

1x 96WP V2 blocking plate

1x 96WP V2 tube holder

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96孔樣品架蓋板

Bertin

S000912-PEVO0-A

each

Well-plate blocking plate for Precellys Evolution

used with well-plate tubes holder for Precellys Evolution.

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多孔板支架

Bertin

S000814-PEVO0-A

each

Well-plate tubes holder for Precellys Evolution

used with well-plate blocking plate for Precellys Evolution.

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Precellys Evolution

維護套件

Bertin

S000808-PEVO0-A

each

Precellys Evolution maintenance kit

Contains:

2   x vacuum joints ;

1   x containment seal ;

1   x anti-rotation tubing kit

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2mL樣品管架蓋板

Bertin

S000802-PEVO0-A

each

Blocking plate for Precellys Evolution

used with 2mL tubes holder for Precellys Evolution

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7mL樣品管架灰色蓋板

Bertin

S000803-PEVO0-A

each

Blocking plate for Precellys Evolution

used with 7mL tubes holder for Precellys Evolution

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15mL樣品管架藍色蓋板

Bertin

S000804-PEVO0-A

each

Blocking plate for Precellys Evolution

used with 15mL tubes holder for Precellys Evolution

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2mL樣品管架

Bertin

S000913-PEVO0-A

each

Tubes holder for Precellys Evolution

with 2mL white blocking plate for Precellys Evolution

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7mL樣品管架

Bertin

S000914-PEVO0-A

each

Tubes   holder for Precellys Evolution

use with 7mL grey blocking plate for Precellys Evolution

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15mL樣品管架

Bertin

S000915-PEVO0-A

each

Tubes   holder for Precellys Evolution

used with 15mL blue blocking plate for Precellys Evolution

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Cryolys Evolution冷卻系統

Bertin

P000671-CLYS2-A

each

Cryolys   Evolution Package

contains   the following items:

Cryolys Evolution module + specific Precellys lid

mounting   screws attached to the Precellys lid

Hinge   reinforcement for the Precellys Evolution

Cable tie

USB stick containing Cryolys Evolution control software

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基本耗材

Precellys Evolution均質器運行基本耗材主要是研磨管、研磨珠或研磨管-研磨珠預混合套裝。

使用Cryolys Evolution制冷單元時,還需消耗干冰。

品名

品牌

貨號

規格

描述

報價

2mL   研磨管

Bertin

P000945-LYSK0-A

500   pieces

2mL   Empty Tubes and Caps with O-ring

2mL管套件含500 個未組裝的管和蓋

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2mL增強研磨管

Bertin

P000943-LYSK0-A

200   pieces

2mL   Reinforced Empty Tubes and Caps

已組裝好管和蓋

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7mL研磨管

Bertin

P000944-LYSK0-A

50   pieces

7mL   Empty Tubes and Caps

管和蓋子,已組裝

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15mL研磨管

Bertin

P000946-LYSK0-A

50   pieces

15mL   Empty Tubes and Caps

管和蓋子,已組裝

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2mL金屬研磨管

Bertin

P002290-LYSK0-A

Each

2mL   Metal tube

for dry grinding of very hard samples(bones, tooth, hair, etc.)

only   compatible with Precellys Evolution.

includes   1 stainless steel tubes

sample   size range: from 20mg to 200mg

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7mL金屬研磨管

Bertin

P002951-LYSK0-A

Each

7mL   Metal tube

for  dry grinding of very hard samples(bones, tooth, hair, etc.)

only   compatible with Precellys Evolution.

includes   1 stainless steel tubes

7mL   sample size range: from 200mg to 1g

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0.1mm氧化鋯珠

Bertin

P000932-LYSK0-A

500   preps

Zirconium   oxide beads in bulk

beads   size: 0.1mm (450g, 500 preps)

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2.8mm氧化鋯珠

Bertin

P000926-LYSK0-A

500   preps

Zirconium   oxide beads in bulk

beads   size: 2.8mm

(325g,   500 preps)

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1.4mm氧化鋯珠

Bertin

P000927-LYSK0-A

500   preps

Zirconium   oxide beads in bulk

beads   size: 1.4mm

(325g,   500 preps)

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5.0mm氧化鋯珠

Bertin

P000930-LYSK0-A

100   beads

Zirconium   oxide beads in bulk

beads   size: 5.0mm

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6.8mm氧化鋯珠

Bertin

P000931-LYSK0-A

100   beads

Zirconium   oxide beads in bulk

beads   size: 6.8mm

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CK14-0.5mL

軟組織勻漿套裝

Bertin

P000933-LYSK0-A

50 preps

Soft   tissue homogenizing CK14-0.5mL Kit

Including:

1.4mm   ceramic beads in 0.5mL standard tubes.

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CK14-2mL軟組織勻漿套裝

Bertin

P000912-LYSK0-A

50   preps

Soft   tissue homogenizing CK14-2.0mL Kit

Including:

1.4mm   ceramic beads in 2mL standard tubes.

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蛋白提取勻漿套裝

CK14-2mL

Bertin

P000973-LYSK0-A

50   preps

Soft   tissue homogenizing CK14-2mL Protein Safe Kit

Including:

1.4mm   ceramic beads in 2mL Protein Safe tubes.

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CK14-7mL軟

組織勻漿套裝

Bertin

P000940-LYSK0-A

50   preps

Soft   tissue homogenizing CK14-7mL Kit

Including:

1.4mm   ceramic beads in 7mL standard tubes

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CK14-WP軟組織勻漿套裝

Bertin

P002137-LYSK0-A


Soft   tissue homogenizing CK14_Well-Plate

Prefilled   96 well-plate

used   with 96WP holder pack V2 for Precellys Evolution

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CK28-2mL

硬組織勻漿套裝

Bertin

P000911-LYSK0-A

50   preps

Hard   Tissue homogenizing CK28-2mL kit

Including:

2.8mm   ceramic beads in standard tubes

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CK28-2mL-R

硬組織勻漿套裝

Bertin

P000916-LYSK0-A

50   preps

Hard   Tissue homogenizing CK28-2mL-R kit

Including:

2.8mm   ceramic beads in reinforced tubes

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CK28-2mL-R

蛋白勻漿套裝

Bertin

P000972-LYSK0-A

50   preps

Hard Tissue homogenizing CK28-2mL-R Protein Safe kit

Including:

2.8mm   ceramic beads

reinforced   tubes

Proteases   inhibitors

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CK28-7mL

硬組織勻漿套裝

Bertin

P000935-LYSK0-A

50   preps

Hard  Tissue homogenizing CK28-7mL kit

Including:

2.8mm   ceramic beads in standard tubes

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CK28-15mL

硬組織勻漿套裝

Bertin

P000947-LYSK0-A

25   preps

Hard   Tissue homogenizing CK28-15mL kit

Including:

2.8mm   ceramic beads in standard tubes

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CKMix-2mL

組織勻漿套裝

Bertin

P000918-LYSK0-A

50   preps

Tissue   homogenizing CKMix - 2mL

Including:

ceramic   mix beads of 1.4mm and 2.8mm in 2mL tubes.

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韌性微生物裂解套裝

VK05 - 0.5mL

Bertin

P000934-LYSK0-A

50   preps

Tough   micro-organism lysing VK05-0.5mL kit

Includes:

0.5mm   glass beads in 0.5mL tubes.

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韌性微生物裂解套裝

VK05 - 2mL

Bertin

P000913-LYSK0-A

50   preps

Tough   micro-organism lysing VK05-2mL kit

includes   50 preps of 0.5mm glass beads in 2mL tubes.

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韌性微生物裂解套裝

VK05 - 15mL

Bertin

P000961-LYSK0-A

25   preps

Tough   micro-organism lysing VK05-15mL kit

Including:

0.5mm   glass beads in 15mL tubes.

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韌性微生物裂解套裝

VK05 WP

Bertin

P002136-LYSK0-A


Tough   micro-organism lysing VK05 prefilled 96 well-plate

for   Precellys Evolution

Prefilled   96 well-plate

used   with 96WP holder pack V2

(S002276-PEVO0-A.0)

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微生物裂解套裝

VK01 - 2mL

Bertin

P000914-LYSK0-A

50   preps

Micro-organism   lysing kit VK01 - 2mL

Including:

0.1mm   glass beads in 2mL tubes.

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微生物裂解套裝

VKMix - 7mL

Bertin

P000937-LYSK0-A

50   preps

Micro-organism   lysing kit VKMix - 7mL

Including:

glass   mix beads of 0.1mm and 0.5mm in 7mL tubes

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細菌裂解套裝

CK01 - 2mL

Bertin

P000919-LYSK0-A

50   preps

Bacteria   lysing kit CK01-2mL

Including:

0.1mm   ceramic beads in 2mL tubes.

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細菌裂解套裝

CK01 - 7mL

Bertin

P000938-LYSK0-A

50   preps

Bacteria   lysing kit CK01-7mL

Including:

0.1mm   ceramic beads in 7mL tubes with O-ring.

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細菌裂解套裝

CK01 - 15mL

Bertin

P000948-LYSK0-A

25   preps

Bacteria   lysing kit CK01-15mL

Including:

0.1mm   ceramic beads in 15mL tubes with O-ring.

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SK38-2mL

土壤樣品研磨套裝

Bertin

P000915-LYSK0-A

50   preps

SK38   Soil grinding kit

Including:

a   mix of glass and ceramic beads in 2mL tubes.

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SK38-7mL

土壤樣品研磨套裝

Bertin

P000936-LYSK0-A

50   preps

SK38   Soil grinding kit

Including:

a   mix of glass and ceramic beads in 7mL tube

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硬組織研磨套裝

MK28 - 2mL

Bertin

P000910-LYSK0-A

50   preps

MK28   Hard Tissue Grinding Kit

Including:

2mL   Tubes with Screw Cap and Skirt

2.8mm   stainless steel beads.

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硬組織研磨套裝

MK28-R - 2mL

Bertin

P000917-LYSK0-A

50   preps

MK28-R   Hard Tissue Grinding Kit

Including:

2mL   Reinforced Tubes with Screw Cap and Skirt

2.8mm   stainless steel beads

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組織研磨套裝

CKMix50-R-2mL

Bertin

P000922-LYSK0-A

50   preps

CKMix50-R   Tissue Grinding Kit

Including:

2mL Reinforced Tubes with Screw Cap, O-Ring and Skirt

A prefilled mix beads of 2.8mm and 5.0mm

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組織研磨套裝

CKMix50-7mL

Bertin

P000939-LYSK0-A

50   preps

CKMix50-7mL   Tissue Grinding kit

Including:

7mL   standard tubes with Screw Cap, O-Ring and Skirt

A   prefilled mix of ceramic mix beads of 2.8mm and 5.0mm

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組織研磨套裝

CKMix50 - 15mL

Bertin

P000949-LYSK0-A

50   preps

CKMix50   Tissue Grinding 15mL kit

Including:

15mL   standard tubes with Screw Cap, O-Ring and Skirt

A  mix of ceramic mix beads of 2.8mm and 5.0mm

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干燥硬組織研磨套裝

CK68-R - 2mL

Bertin

P000923-LYSK0-A

50   preps

Dry hard tissue grinding CK68 2mL-R kit

including:

prefilled ceramic beads of 6.8mm in 2mL reinforced tubes

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硬組織研磨套裝

CK68 - 15mL

Bertin

P000950-LYSK0-A

25   preps

Dry hard tissue grinding CK68 15mL kit

including:

prefilled ceramic beads of 6.8mm in 15mL tubes

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樣品研磨套裝

GK60 - 2mL

Bertin

P000924-LYSK0-A

50   preps

Sample   grinding GK60 - 2mL

Including:

garne flakes 0.7mm beads and one Ceria-stabilized ceramic bead

2mL   reinforced tubes

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訂貨指南

Precellys 24 Touch組織均質器的出廠配置不含研磨管、研磨珠等耗材。

針對實驗室樣品材料處理體積、材料質地和均質技術要求,應選擇與樣品體積大小、類型相匹配的管子和與處理任務匹配的研磨珠,以實現可靠、高效和穩定處理效果。

品名

品牌

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規格

描述

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Precellys Evolution均質器

標準套裝

Bertin

P000062-PEVO0-A.0

each

Precellys Homogenizer Standard package

Including:

Precellys   Evolution with 2mL holder pack

toric joint and containment seal

power cable

spare toric joint

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Precellys Evolution均質器

7mL套裝

Bertin

P000062-PEVO0-A07

each

Precellys Evolution Homogenizer 7mL package

for   2mL or 7mL tubes

Including:

Precellys Evolution Standard package(P000062-PEVO0-A.0)

7mL tubes holder pack (S000911-PEVO0-A)

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Precellys Evolution均質器

15mL套裝

Bertin

P000062-PEVO0-A15

each

Precellys Evolution Homogenizer 15mL package

to   process 2mL or 15mL tubes

Including:

Precellys Evolution Standard package(P000062-PEVO0-A.0)

15mL tube holder pack (S000810-PEVO0-A)

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Precellys Evolution均質器

完整套裝

Bertin

P000062-PEVO0-AFU

each

Precellys Evolution Homogenizer Full package

to   process 2mL or 7mL or 15mL tubes.

Including:

Precellys Evolution Standard package(P000062-PEVO0-A.0)

7mL tubes holder pack (S000911-PEVO0-A)

15mL  tube holder pack (S000810-PEVO0-A)

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Precellys Evolution均質器

2mL低溫套裝

Bertin

K002198-PEVO0-A0

each

Combo Precellys Evolution + Cryolys Evolution

Including:

Precellys Evolution Standard package(P000062-PEVO0-A.0)

Cryolys Evolution Package (P000671-CLYS2-A)

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Precellys 均質器

7mL低溫套裝

Bertin

K002198-PEVO0-A07

each

Precellys Evolution Homogenizer 7mL package

Including:

Precellys   Evolution /Cryolys Evolution pack (K002198-PEVO0-A0)

7mL tubes holder pack (S000911-PEVO0-A)

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