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小型蛋白垂直電泳槽

伯樂Mini-Protean Tetra蛋白電泳槽

伯樂Mini-Protean Tetra小型蛋白電泳槽可容納1~4塊小型手灌膠或Ready Gel 預制膠工作,用于蛋白SDS-PAGE垂直電泳分離。本欄目提供Mini-Protean Tetra的組成及選型配置方案。

設備名稱:蛋白垂直電泳槽  蛋白垂直電泳儀
制 造 商:美國Bio-Rad公司           產 地:新加坡/中國
品    牌:伯樂(Bio-Rad)    
型    號:Mini-Protean Tetra
電泳配件:見[主要附件]  商品價格:見[訂貨指南]

主要功能用途

Bio-Rad Mini- Protean Tetra蛋白垂直電泳槽,是Western Blot實驗流程重要組成部件,可容納1~4 塊小型手灌膠或Ready Gel 預制膠(10×8.3cm)的電泳,主要用于蛋白聚丙烯酰胺凝膠電泳(PAGE或SDS-PAGE)實驗。

 

性能特點

伯樂Mini-Protean Tetra蛋白垂直電泳槽,兼容Mini-Protean precast gels預制凝膠、Ready Gel precast gels預制凝膠或手工自制PAGE凝膠。

可在一小時內完成多達四塊小型蛋白凝膠的電泳分離。

軟橡膠墊片與玻璃板形成嚴密密封,確保無泄漏灌膠。

帶有簡單凸輪的灌制框,確保在任何平面上實現精確對齊。

平行排列的灌膠架,能同時灌制兩塊凝膠,彈簧杠桿設計使得軟橡膠沉淀產生良好的密封性。

封邊墊條的長玻板加厚,不易破碎。

標有厚度和孔數的玻板和加樣梳便于識別。

上樣向導防止泳道遺漏上樣或重復上樣。

可互換模塊可將Mini- Protean Tetra電泳槽轉換為可進行Western轉印的 Mini Trans-Blot轉印槽。

 

 

主要技術規格

伯樂Mini-Protean Tetra蛋白垂直電泳槽適用的凝膠類型包括:Mini- Protean預制膠、手灌膠

凝膠數:1–4塊

凝膠格式:小型

適用的凝膠尺寸(寬×長)

預制膠:8.6×6.8cm;

手灌膠:8.3×7.3cm

玻璃板尺寸:短板10.1×7.3cm,玻板10.1×8.2cm

緩沖液運行需求

運行2塊凝膠緩沖液總體積:700mL

運行4塊凝膠緩沖液總體積:1000 mL

SDS-PAGE 典型運行時間:35–45 分鐘(在200V恒定電壓下)

Mini-PROTEAN Tetra加樣梳每孔最大上樣量

1.0mm 10孔梳:44 μL

1.0mm 15孔梳:26 μL

 

安裝運行條件

相對濕度范圍:0~95% R.H.

工作環境溫度:0℃~40℃

外觀尺寸(寬×長×高):12×16×18cm

Mini- Protean Tetra 電泳槽重量:1.0kg

(電泳電源另配)

 

組成配置

電泳槽系統

伯樂Mini-Protean Tetra蛋白電泳槽全套工作裝置包括電泳緩沖液槽電極組件制膠組件輔助工具耗材。
按一次可運行的最大凝膠數量,分為4-gel、2-gel兩種規格。
如需跑4塊凝膠,可訂購4凝膠槽,也可在2凝膠套裝基礎上加配Mini-Protean Tetra相應功能附件,擴展為4塊凝膠工作通量。

此外,根據使用的凝膠來源類型,還可單獨配置伯樂預制膠專用套裝。

品名

品牌

貨號

規格

描述

報價

蛋白垂直電泳槽

(4-gel/10-well/1.0mm)

Bio-Rad

1658001

1套

Mini-PROTEAN Tetra Cell

4-gel system, 1.0mm gel thickness

includes:

Tank, lid with power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 1.0mm combs

5×gel releasers(1653320)

詢價

詢價

蛋白垂直電泳槽

(4-gel/10-well/1.5mm)

Bio-Rad

1658006

1套

Mini-PROTEAN Tetra Cell

4-gel system, 1.5mm gel thickness

includes:

Tank, lid, power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 1.5mm combs

5×gel releasers(1653320)

詢價

詢價

蛋白垂直電泳槽

(4-gel/10-well/0.75mm)

Bio-Rad

1658000

1套

Mini-PROTEAN Tetra Cell

4-gel system, 0.75mm gel thickness

includes:

Tank, lid, power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 0.75mm combs

5×gel releasers(1653320)

詢價

詢價

蛋白垂直電泳槽l

(2-gel/10-well/1.0mm)

Bio-Rad

1658003

1套

Mini-PROTEAN Tetra Cell

2-gel system, 1.0mm gel thickness

includes:

1×casting stand, 2×casting frames;

5×10-well combs;

5 short plates and 5 spacer plates

詢價

詢價

蛋白垂直電泳槽

(2-gel/10-well/1.5mm)

Bio-Rad

1658007

1套

Mini-PROTEAN Tetra Cell

2-gel system, 1.5mm gel thickness

includes:

1×casting stand, 2×casting frames;

5×10-well combs;

5 sets of glass plates

詢價

詢價

蛋白垂直電泳槽

(2-gel/10-well/0.75mm)

Bio-Rad

1658002

1套

Mini-PROTEAN Tetra Cell

2-gel system, 0.75 mm gel thickness

includes:

1×casting stand, 2×casting   frames;

5×10-well combs;

5 short plates and 5 spacer plates

詢價

詢價

Mini Precast Gels(4-gel)

預制膠蛋白電泳槽

Bio-Rad

1658004

1套

Mini-PROTEAN Tetra Vertical Electrophoresis Cell

for Mini Precast Gels, 4-gel

includes:

electrode assembly;

companion running module;

tank, lid with power cables, cell buffer   dam

詢價

詢價

Mini Precast Gels (2-gel)

預制膠蛋白電泳槽

Bio-Rad

1658005

1套

Mini-PROTEAN Tetra Vertical Electrophoresis  Cell

for Mini Precast Gels, 2-gel

includes:

electrode assembly;

tank, lid with power cables, cell buffer dam

詢價

詢價



電泳槽組件

伯樂Mini-Protean Tetra 小型蛋白垂直電泳槽的制膠模塊(Casting Module),是指用于灌制特定厚薄和齒孔數目蛋白凝膠所需的模塊化套裝。

模塊由通用核心組件(Core System)和差異化功能組件組成。通用核心灌膠組件為灌膠架(含4個密封墊),夾膠框(casting frames)和或膠鏟(gel releaser)。差異化功能組件則包括特定規格的玻璃板套件,電泳梳和上樣導槽。

伯樂Mini-PROTEAN Tetra Cell小型垂直電泳槽有多種灌膠模塊可選,分別滿足不同上樣量和對凝膠厚度的特殊要求。

品名

品牌

貨號

規格

描述

報價

1.0mm/10孔 制膠模塊

Bio-Rad

1658015

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well/1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

1.0mm/15孔制膠模塊

Bio-Rad

1658016

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well, 1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

1.0mm/5孔 制膠模塊

Bio-Rad

1658013

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates.

詢價

詢價

1.0mm/9孔 制膠模塊

Bio-Rad

1658014

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide.

詢價

詢價

1.5mm/10孔 制膠模塊

Bio-Rad

1658021

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well, 1.5 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

1.5mm/15孔 制膠模塊

Bio-Rad

1658022

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well, 1.5 mm gel casting module

includes:

2×casting stands, 4×casting frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

1.5mm/5孔 制膠模塊

Bio-Rad

1658019

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   1.5 mm gel casting module

includes   the following componets:

2   casting stands, 4 casting frames

5   combs, 5 short plates and 5 spacer plates.

詢價

詢價

1.5mm/9孔 制膠模塊

Bio-Rad

1658020

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   1.5 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

0.75mm/10孔 制膠模塊

Bio-Rad

1658010

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well,   0.75 mm gel casting module

includes   the following componets:

2   casting stands, 4 casting frames

5   combs, 5 short plates and 5 spacer plates

sample   loading guide.

詢價

詢價

0.75mm/15孔 制膠模塊

Bio-Rad

1658011

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

0.75mm/5孔 制膠模塊

Bio-Rad

1658008

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

0.75mm/9孔 制膠模塊

Bio-Rad

1658009

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

灌膠通用套件(4膠)

Bio-Rad

1658051

1套

Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit

includes:

2×stands, 4×frames,4×gaskets;

5×gel   releasers.

適用于4膠Mini-Protean Tetra電泳槽

詢價

詢價

灌膠通用套件(2膠)

Bio-Rad

1658052

1套

Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit

includes:

1×stand, 2×frames, 2×gaskets;

5×gel releasers.

適用于2膠Mini-Protean Tetra電泳槽

詢價

詢價

基本耗材

伯樂Mini-Protean Tetra垂直電泳槽工作的基本耗材主要包括蛋白凝膠玻璃板等。

品名

品牌

貨號

規格

描述

報價

1.0mm厚玻璃板

Bio-Rad

1653311

5塊/包

Spacer Plates with 1.0 mm Integrated Spacers

帶1.0mm厚封邊條,為1.0mm凝膠灌膠組件。

詢價

1.5mm厚玻璃板

Bio-Rad

1653312

5塊/包

spacer plate   with 1.5 mm integrated spacer

帶1.5mm厚封邊條,用于1.5mm凝膠的制備

詢價

薄玻璃板

Bio-Rad

1653308

5塊/包

short plate for Mini-PROTEAN Tetra System

 and Mini-PROTEAN 3 Multi-Casting Chamber.

外形尺寸(W×L):101× 73 mm

與帶任一厚度封邊墊條的長玻璃板配套使用

詢價

 

訂貨指南

1、蛋白電泳槽的選型

因不同實驗樣品樣品數量、蛋白類型和表達豐度、實驗經驗與操作習慣的不同,選擇蛋白電泳槽重點要結合所用凝膠的厚度(0.75mm、1.0mm、1.5mm)、每張凝膠電泳的上樣孔數(常用的10孔-最大上樣量66μl、15孔-40μl)、每輪電泳的凝膠通量(4膠、2膠)和凝膠類型(手灌膠、預制膠)來確定具體選型方案。

如單獨訂購Mini-Protean Tetra小型蛋白垂直電泳槽或更換電泳灌膠配件,請請參考本欄目“組成配置”“主機”部分所列各型電泳槽的技術規格和詳細配置內容。最常用的1658001槽單元,其標準配置適用的是1.0mm/10-well/4-gel凝膠。

 

2、蛋白電泳槽電源的選擇

伯樂Mini-Protean Tetra小型蛋白垂直電泳槽本身工作運行對電源并無特殊要求。伯樂PowerPac Basic 、PowerPac HC、PowerPac Universal等多個型號均可滿足同時給1-4個電泳槽供電的要求。

但須注意的是,通常情況下,蛋白電泳槽只是Western Blot實驗流程的一個功能組件,單個電泳槽輸出凝膠數量(2塊或4塊)與電轉印槽(2塊)各自最大凝膠工作通量不同,要充分考慮實驗流程上的有效銜接問題。

特別是當實驗樣品數量多于4塊凝膠的情況下,根據自身實驗條件和經驗,通盤考慮電轉印槽、電轉印模塊的配置數量。

與常規140V左右恒壓蛋白電泳跑膠不同,電轉印對電流要求較高。通常,每塊凝膠-印跡膜的工作電流可達200mA甚至更高。若同時進行4塊凝膠轉膜操作,單一Basic電源很可能無法滿足供電要求。故需考慮HC、PowerPac Universal等具有電流輸出能力大于0.8A的電源型號確有必要。

 

3、電泳槽與與Mini-Trans Blot小型蛋白電泳轉印槽搭配

電轉移槽的選型配置問題,請參考Mini-Trans Blot小型蛋白轉印槽欄目內容。


序號

品名

貨號

套裝配置描述

報價

1-1

Basic蛋白電泳套裝

(1.0mm/10-well/4-gel)

1658025

Mini-PROTEAN Tetra and Power Supply Bundle

includes:

Mini-PROTEAN Tetra 4-gel system (1658001);

PowerPac Basic power supply (1645050).

詢價

詢價

1-2

HC蛋白電泳套裝

(1.0mm/10-well/4-gel)

1658027

Mini-PROTEAN Tetra and Power Supply Bundle

includes:

Mini-PROTEAN Tetra 4-gel system (1658001);

PowerPac HC power supply (1645052).

詢價

詢價






2-1

電泳-轉印槽組合裝

(1.0mm/10-well/4-gel/2-blot)

1658029

Electrophoresis and blot Bundle (10-well/1.0mm/4-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658001);

Mini Trans-Blot Module (1703935).

詢價

詢價

2-2

Basic電源-電泳-轉印套裝

(1.0mm/10-well/4-gel/2-blot)

1658033

Electrophoresis, blot and Power Supply   Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

2-3

HC電源-電泳-轉印套裝

(1.0mm/10-well/4-gel/2-blot)

1658035

Electrophoresis, blot and Power Supply Bundle

for 10-well, 1.0 mm, 4-gel system

includes:

Mini-PROTEAN Tetra Cell (1658001);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052)

詢價

詢價

2-4

HC電源-電泳-轉印套裝

(1.0mm/10-well/4-gel/4-blot)

1658035-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini Trans-Blot Transfer Cell (1703930);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價

2-5

HC電源-電泳-轉印高級套裝

(1.0mm/10-well/4-gel)

(1.5mm/10-well/4-gel)

(4-blot)

1658035-U

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini-PROTEAN Tetra Cell Casting Module (1658021);

Mini Trans-Blot Cell (1703930);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價






3-1

電泳-轉印槽組合裝

(1.5mm/10-well/2-gel/2-blot)

16580007-B

Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

includes:

Mini-PROTEAN   Tetra Cell for handcast 2-gel (1658007);

Mini Trans-Blot Module (1703935).

詢價

詢價

3-2

電泳-轉印組合裝

(1.5mm/10-well/4-gel/2-blot)

16580006-B

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

詢價

詢價

3-3

Basic電源-電泳-轉印套裝

(1.5mm/10-well/2-gel/2-blot)

16580007-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

3-4

Basic電源-電泳-轉印套裝

(1.5mm/10-well/4-gel/2-blot)

16580006-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEA Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

3-5

HC電源-電泳-轉印套裝

(1.5mm/10-well/4-gel/4-blot)

16580006-U

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

Mini Trans-Blot Cell (1703930)

PowerPac HC Power Supply (1645052).

詢價

詢價






4-1

電泳-轉印槽組合裝

(1.0mm/10-well/2-gel)

1658003-C

Electrophoresis and blot Bundle (10-well/1.0mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658003);

Mini Trans-Blot Module (1703935).

詢價

詢價

4-2

Basic電源-電泳-轉印套裝

(1.0mm/10-well/2-gel/2-blot)

1658003-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658003);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

4-3

電泳-轉印槽組合裝

(1.5mm/10-well/2-gel)

1658007-C

Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658007);

Mini Trans-Blot Module (1703935).

詢價

詢價

4-4

Basic電源-電泳-轉印套裝

(1.5mm/10-well/2-gel/2-blot)

1658007-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658007);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價






5-1

蛋白預制膠

電泳-轉印槽組合

1658030

Vertical Electrophoresis and Mini Trans-Blot Module Bundle

for Mini Precast 4-Gel

includes:

4-gel Mini-PROTEAN Vertical Cell (1658004);

Mini Trans-Blot Module (1703935).

詢價

詢價

5-2

蛋白預制膠

Basic電源電泳轉印套裝

1658034

Electrophoresis, blot and Power Supply Bundle

for precast 4-gel

includes:

Mini-PROTEAN Tetra Cell for Mini Precast Gels (1658004);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

5-3

蛋白預制膠

HC電源電泳轉印套裝

1658036

Electrophoresis, blot and Power Supply Bundle

includes:

for Mini Precast Gels, 4-gel

Mini-PROTEAN Tetra Cell (1658004);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價

(更多配置方案詳情請咨詢客服)